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Carbon dioxide metabolism in Streptococcus thermophilus : phyisological and ecological importance, and dairy applications : phd in food biotechnology : tesi di dottorato

Arioli, Stefania <1975- >

Tesi o dissertazioni - 2009

Abstract
Carbon dioxide metabolism in Streptococcus thermophilus: physiological and ecological importance and dairy applications We investigated the role and the influence in the carbon dioxide and energetic metabolism of S. thermophilus DSM 20617T of the two main CO2-consuming pathways governed by the phosphoenolpyruvate carboxylase (PPC) and the carbamoylphosphate synthetase (CPS), which catalyze the fixation of carbon dioxide to phosphoenolpyruvate and glutamine, respectively. We highlighted the relevance of both PPC and CPS activities for an optimal growth and adaptation of this bacterial species in milk environment. Moreover we investigated the effect of a limited carbon dioxide availability for S. thermophilus, resulting in a strong alteration in cell morphology. The comparative proteomic analysis carried out between cultures grown under enriched CO2 or under N2 suggested that carbon dioxide stimulates energetic metabolism. Finally, we studied the impact on the energetic metabolism of urease, a CO2-producing enzyme which is peculiar of S [...]
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Inventario
3818
Collocazione
D. 2009 0055
Note
Autorizzazione: si.