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Development of a novel nanosensor for the study of biomolecular interactions : dottorato di ricerca in chimica del farmaco

Morasso, Carlo Francesco <1979- >

Tesi o dissertazioni - 2008

The present work is focused on the development of a novel qualitative and quantitative label-free technique enabling detailed measurement of ligand–receptor interactions occurring on the surface of colloids. The key factor is the use of receptor-coated nanospheres matched in refractive index with water and therefore optically undetectable (‘‘phantom’’) when not involved in adhesion processes. At the occurrence of ligand binding at the receptor sites, optically unmatched material adsorbs on the nanoparticle surface, giving rise to an increment in their scattering cross section up to a maximum corresponding to saturated binding sites. The analysis of the scattering growth pattern enables extracting the binding affinity. This label-free method has been assessed through the precise determination of the binding constant of the antibiotic Vancomycin with the tripeptide L-Lys-D-Ala-D-Ala and has been successfully employed to study protein-protein and enzyme-substrate interactions. An implementation [...]
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