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Analisi del profilo di espressione dei geni dell'apoptosi nelle LLC mediante microfluidic cards : dottorato di ricerca in ematologia sperimentale : tesi di dottorato

Pasquini, Maria Cristina <1976- >

Tesi o dissertazioni - 2009

Many evidences suggest a deregulation of the apoptosis in CLL but only a few studies focused on the quantitative expression of cell cycle and apoptosis associated genes. In order to investigate the molecular mechanisms that regulate apoptosis in CLL, in this study we examined the apoptosis related genes expression profile of CLL using MicroFluidic Card™ low density array. Fifty-five CLL patients with a median age of 64 years (range 42-77 years) entered in the study. Peripheral blood was collected from all the patients as well as from 30 health donor. None of the enrolled patients underwent therapy for CLL before sample collection. Blood mononuclear cells were prepared using Ficoll- IsopaqueTM density centrifugation and CD19-positive B cells were isolated with appropriate beads by magnetic cell separation (Mylteni Biotech, Auburn, CA).Ninety-two apoptosis-related genes and 4 reference genes were analyzed in duplicate by MicroFluidic Card (MCF) method. Five hundred nanograms per port of cDNA [...]
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