opac, catalogo, online,servizi, biblioteca, sebina

January, 2009 : analysis of protein complexes by chemical cross-linking and mass spectrometry : phd in molecular medicine

Maiolica, Alessio <1980- >

Tesi o dissertazioni - 2009

Abstract The aim of the present thesis lies in the study of protein complexes by mass spectrometry. We report the results of a combined approach of cross-linking, mass spectrometry, and bioinformatics relative to the analyses of three human complexes: the Eps8-Actin dimer, the Ndel1 homodimer and the Ndc80 heterotetramer. An important limitation of the cross-linking approach, so far, was the identification of cross-linked peptides from fragmentation spectra. Our novel approach overcomes the data analysis bottleneck of cross-linking and mass spectrometry. We have constructed a purpose-built database to match spectra with cross-linked peptides, defined a score that expresses the quality of our identifications, and estimated false positive rates. Our analysis has shed light on critical structural parameters such as the mechanism of binding of Eps8 to actin, the directionality of the homo-dimeric coiled-coil of Ndel1 and the register of the hetero-dimeric coiled-coils of the Ndc80 complex. [...]
  • Lo trovi in
  • Scheda
  • Unimarc