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Mechanisms controlling UvrD helicase activities in homologous recombination : phd degree in molecular medicine

Saponaro, Marco <1980- >

Tesi o dissertazioni - 2009

Abstract Homologous Recombination is a DNA damage repair pathway that engages with Double Strand Breaks and the gaps that arise during DNA replication. Inappropriate recombination however can lead to genome instability, for this reason homologous recombination has to be tightly regulated. The UvrD DNA helicase family is involved in the regulation of the recombination processes. In particular UvrD helicases can disrupt Rad51 nucleofilaments antagonizing inapt recombination. Srs2, the member of the UvrD family in S. cerevisiae, is phosphorylated in DNA damaging conditions by the cyclin dependent kinase CDK1 and contains seven putative phosphorylation sites. CDK1 is a key regulator of the recombination process, although most of the targets are unknown. The purposes of this phosphorylation of Srs2 were unknown, therefore we have created two mutants of Srs2 that mimic the unphosphorylated and the phosphorylated forms of Srs2, in order to better characterize the modification. With these phosphorylation [...]
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