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Identification of novel genes involved in acute myeloid leukemogenesis using gene expression profiling : phd degree in molecular medicine

Scafetta, Gaia <1978- >

Tesi o dissertazioni - 2009

ABSTRACT Acute myeloid leukemia is sustained by a group of cells, known as leukemic stem cells (LSC) that resemble normal hematopoietic stem cells (HSC) but present alterations in their growth properties and increased cell survival. The persistence of LSC after cytotoxic and antiproliferative therapy is thought to be responsible for disease relapse, which occurs in approximately 70% of AML cases. The molecular analysis of LSC will likely lead to novel therapeutical strategies aimed at eradicating the disease. In an effort to characterize specific features of leukemia initiating cells we decided to directly assess their transcriptional status in two different murine AML models, and compare their global gene expression profile to that of the normal counterparts. Leukemic mice were generated by retroviral transduction of bone marrow cells with PML/RARalfa or AML1/ETO oncogenes. Different cellular subpopulations were purified using antibodies against specific surface markers (Mac-1, c-kit and [...]
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