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Synthesis of oligosaccharides and glycoconjugates as antigens for vaccine formulation : dottorato di ricerca in scienze chimiche : tesi di dottorato

Buzzi, Benedetta <1979- >

Tesi o dissertazioni - 2008

SYNTHESIS OF OLIGOSACCHARIDES AND GLYCOCONJUGATES AS ANTIGENS FOR VACCINE FORMULATION N. meningitidis type A (MenA) is a Gram-negative encapsulated bacterium which is the main responsible for epidemics in the sub-saharian Africa. The development and manufacture of an efficient glycoconjugate vaccine against MenA is greatly hampered by the poor hydrolytic stability of its capsular polysaccharide. Since the chemical lability derives from the instability of the phosphodiester bridges, I synthesised the phosphono analogue of the dimer and the trimer of N. meningitidis type A CPS. The immunogenecities of the synthetic molecules have been investigated by a competitive ELISA assay. Further aim of this work was the synthesis of multivalent neo-glycoconjugates. In particular, using a squarate linkage, I synthesised the neo-glycoconjugate between PAMAM-4 and PAMAM-8 with the monomer fragment of MenA. Following the same synthetic strategy (squarate linkage), oligomers were also coupled with HSA. The number of oligosaccharide units incororated was determined by MALDI-TOF mass [...]
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D. 2009 0347 /1-3
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